Aims:This study evaluated semi-professional football players’ awareness of dental injuries and prevention, focusing on mouthguard use and its association with injury experience, with the aim of proposing new guidelines for enhanced prevention strategies. Instrument & Methods:This cross-sectional survey of 212 male players with a mean age of 22.5±2.5 years covered demographics, injury history, emergency procedures (including tooth replantation), and mouthguard usage. The questionnaire used assessed the level of awareness of dental injuries and their prevention among football players. Data were analyzed using descriptive statistics and chi-square tests. Findings:Only 59.90% of participants knew about tooth replantation, with just 14.15% identifying the 60-minute critical window. While 69.34% recognized the importance of immediate action, mouthguard use was extremely low (4.25%). The main reasons for non-use were perceived unnecessity (41.98%), communication issues (23.11%), and breathing difficulties (19.81%). Only 5.19% of participants reported organized team dental care. Players with injury experience showed higher awareness and a greater inclination toward mouthguard use. Conclusion:Football players have insufficient awareness of dental injury prevention and very low mouthguard use.