Aims: Despite the widespread use of information technology in healthcare, nurses’ awareness and understanding of nursing informatics as a distinct field remain limited. This study evaluated nurses’ knowledge of nursing informatics and their views on establishing it as an independent discipline. Instrument & Methods: This cross-sectional qualitativestudy was conducted in two phases. First, interviews with hospital supervisors affiliated with medical sciences universities in Mashhad explored nursing informatics and its applications. In the second phase, based on these interviews and a literature review, a questionnaire was developed and electronically distributed to 1,440 nurses in Iran. Content validity was confirmed by four medical informatics experts, and reliability was high (Cronbach’s alpha=0.94). Findings: Nurses’ knowledge level regarding nursing informatics was measured at 43.32%, while their significant relationship toward nursing informatics amounted to 74.82%. There was no significant relationship between perspective and demographic factors, as well as between awareness level and gender (p>0.05). However, a significant relationship was observed among age, educational attainment, organizational position, employment status, and employment history parameters with the awareness level of nursing informatics (p<0.05). Conclusion: Half of the nurses in Iran believe in the feasibility of establishing a nursing informatics field.