en <span style=font-size:12pt><span style=font-family:Times New Roman,serif><b><span style=font-size:10.0pt><span style=font-family:Cambria,serif><span style=color:#4f81bd>Aims:</span></span></span></b><span style=font-size:10.0pt><span style=font-family:Cambria,serif> Hospital-acquired infections represent a significant global health challenge, leading to increased patient morbidity, mortality, and healthcare costs. This study aimed at evaluating the knowledge and practices of healthcare workers at Ghaem Hospital’s special care units concerning the control of hospital-acquired infections. </span></span></span></span><br> <span style=font-size:11pt><span style=line-height:normal><span style=font-family:Calibri,sans-serif><b><span style=font-size:10.0pt><span style=font-family:Cambria,serif><span style=color:#4f81bd>Instrument & Methods:</span></span></span></b><span style=font-size:10.0pt><span style=font-family:Cambria,serif> This descriptive-analytical research employed a comprehensive approach, encompassing all nursing and patient-care staff in the special care units of Ghaem Hospital in Mashhad. Conducted from 2022 to 2023, the study utilized a census and sampling method to gather data, which were collected via two questionnaires-one capturing demographic details and the other assessing knowledge and practices related to infection control.</span></span></span></span></span><br> <span style=font-size:11pt><span style=line-height:normal><span style=font-family:Calibri,sans-serif><b><span style=font-size:10.0pt><span style=font-family:Cambria,serif><span style=color:#4f81bd>Findings:</span></span></span></b><span style=font-size:10.0pt><span style=font-family:Cambria,serif> A total of 96 individuals participated in the study, predominantly nurses (62.5%), followed by paramedics (24%), and doctors (13.5%). Practices of hand hygiene before procedures varied in different groups. 30% of doctors, 34% of nurses, and 30% of paramedics thoroughly washed all parts of their hands; 63% of doctors, 57% of nurses, and 50% of paramedics used soap; complete fingernail washing was done by 43% of doctors, 45% of nurses, and 56% of paramedics, showing a statistically significant difference (p<0.05). Post-procedure hygiene responses were positive from 44% of doctors, 33% of nurses, and 50% of paramedics. Knowledge regarding the transmission of urinary and ventilator-associated respiratory infections was generally high among doctors and nurses but lower among paramedics.</span></span></span></span></span><br> <span style=font-size:11pt><span style=line-height:normal><span style=font-family:Calibri,sans-serif><b><span style=font-size:10.0pt><span style=font-family:Cambria,serif><span style=color:#4f81bd>Conclusion:</span></span></span></b><span style=font-size:10.0pt><span style=font-family:Cambria,serif> Healthcare staff adheres to infection control guidelines to varying degrees, with doctors and nurses generally performing better than paramedics.</span></span></span></span></span><br> Infectious Disease Transmission, Patient-to-Professional,Health Personnel,Hand Hygiene,Infection Control, 159 164 http://hehp.modares.ac.ir/browse.php?a_code=A-10-79541-1&slc_lang=en&sid=4 B. Khodadadi 1003194753284600326075 1003194753284600326075 No Department of Nursing, Mashhad Branch, Islamic Azad University, Mashhad, Iran M. Sharafi 1003194753284600326076 1003194753284600326076 No Department of Anesthesiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran M.R. Modarres Moghaddam 1003194753284600326077 1003194753284600326077 No Department of Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran B. Fazli 1003194753284600326078 1003194753284600326078 Yes Department of Anesthesiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran