en <div style=text-align: justify;><strong>Aims:</strong> With age, a person faces many threats, such as developing chronic diseases. Health literacy plays a crucial role in the prevention and treatment of these diseases. Therefore, the present study was conducted to determine the relationship between health literacy and multi-infection based on gender differences in the elderly.<br> <strong>Instrument & Methods:</strong> This cross-sectional study was performed on the elderly population over 60 years in Comprehensive Health Center and Health Post at Malekan City, East Azerbaijan Province, Iran, in 2018. Four hundred eighty-seven people were sampled by the stratified random sampling method. Demographic and standard health literacy questionnaires were used to collect data. Data were analyzed using an independent t-test, chi-square, and linear regression model by SPSS 26 software.<br> <strong>Findings:</strong> The mean participants’ age was 64.9±6.5 years. The health literacy level of 63.86% of the participants was insufficient, and only 1.23% of the subjects had excellent health literacy. The rate of multi-infection in women was significantly higher than in men.<br> <strong>Conclusion: </strong>The level of health literacy and multi-infection rate in women is higher than in men.</div> Health Literacy,Multi-infection,Gender Differences,Elderly, 105 110 http://hehp.modares.ac.ir/browse.php?a_code=A-10-26054-11&slc_lang=en&sid=4 Sh. Papi 1003194753284600210611 1003194753284600210611 Yes Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran Z. Karimi 1003194753284600210612 1003194753284600210612 No Department of Health Education and Promotion, School of Health, Qom University of Medical Science, Qom, Iran F. Saadat Talab 1003194753284600210613 1003194753284600210613 No Iranian Research Center on Aging, University of Social Welfare & Rehabilitation Science, Tehran, Iran F. Hosseini 1003194753284600210614 1003194753284600210614 No Department of Epidemiology & Biostatics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran H. Afrouzeh 1003194753284600210615 1003194753284600210615 No Faculty of Humanities, Science and Research Branch, Islamic Azad University, Tehran, Iran M. Yousefi 1003194753284600210616 1003194753284600210616 No Department of Biomedical Engineering, School of Technical and Engineering, Dezful Branch, Islamic Azad University, Dezful, Iran S. Norouzi samaneh.norouzi1993@gmail.com 1003194753284600210617 1003194753284600210617 No Department of Health Education and Health Promotion, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran